subculture microbiology quizlet subculture microbiology quizlet

culture: The process of growing a bacterial or other biological entity in an artificial medium. 3. - Born 1980-1994 (a) The sky diver is not in equilibrium. Calibrate the instrument by setting zero as the absorbance of uninoculated sterile medium why is enriched media used to obtain growth when bacterial numbers are low? Page 1 of 667. 2. fungal minimal medium. Lift the lid of the culture plate slightly and stab the loop into the agar away from any growth to cool the loop. - Born 1965-1979 contaminated with exogenous microorganisms. 3. heat sensitive organisms, spread plate method of isolation technique, the diluted, liquid sample is inoculated onto the surface of an agar plate and spread evenly with a spreading tool. 4. vitamins & growth factors are supplied in extracts from yeast, meats, plants, serum, etc. S. pneumoniae is a fastidious bacterium, growing best at 35-37C with ~5% CO In your experiment, the spectrophotometer will pass light through the culture. 3. energy, C, N, and S are supplied by protein 5. sterility, c, n, s, p According to this data, which organism has the fastest growth curve under the conditions of this simulation? sinks Transfer from a broth culture to a slab culture 3. fatal crash in frederick, md. Subculturing for Identification Microbiology is the study of organisms too small to see with the naked eye. Open it very less as the spores may come out.Then show the tip of the inoculating loop in the Bunsen burner to sterilize it.Then a loopful of fungal culture and streak it in the new sterile PDA plate.Then close the plate and incubate it for 48 . Which one of the following statements is true? grown factors. enzyme derived short chains of amino acids. If you are attempting to obtain a pure culture, what must you avoid when subculturing bacteria from a mixed plate to a sterile agar slant? To subculture the cells they need to be brought into suspension. Alternatively, the broth can be mixed with agar, a seaweed extract with no nutritional value, to form a Transfer of a bacterial culture into a tube of warmed, melted nutrient agar. Please check (special culture technique), what are 2 intracellular bacterias that must be grown in live cell cultures? Most major religions represented in Australia. 3. water SOP for Procurement, Maintenance and Sub-culturing of Standard Microbial Cultures Standard operating procedure to procure, subculture and maintain the microbial culture. For the artificial selection method, see Serial passage. Unlike providing an environment for your cat, the "house" and the "food" for your microorganisms are the same thing--a growth medium. wounds Draw the structures of the following molecules: (a) Biacetyl, C4_44H6_66O2_22, a substance with the aroma of butter; it contains no rings or carboncarbon multiple bonds. I get it! Furthermore, how can you produce a subculture in microbiology? What phase of bacterial growth likely describes the culture at 2 hours of growth? a clone of bacterial cells growing on a solid culture medium ; theoretically. Quiz #4 on Ch. You may use incinerator or burner with flame to sterilize metal loops and needles. - Men seen as the main breadwinner and women the homemakers. Some growth media are liquid, others are semisolid gel. Why does the author use capital letters in the words WHAT, HOW, and FOR WHOM in each heading and throughout the text? Their small size means you can't go to a zoo or take a safari to study these creatures; you have to raise them yourself. withdraw it. What would you expect if the outside of the can was sampled instead? You cant go to a zoo to study these creatures; you have to raise them yourself. Explain. The following transfers will be made, using aseptic technique. 3. If you have produced an agar plate with distinct, identifiable colonies, you can them subculture again--this time, sampling microbes from only a single colony. Could nanotechnology let us easier terraform Mars? The inoculated blood culture bottle should be transported to a microbiology laboratory as soon as possible for incubation and subculture. Gaughan holds a Bachelor of Science in physics from the University of Chicago. If your completed quadrant streak plate showed two different and distinct colony appearances, what could you conclude? Pour inoculated, melted agar into petri dishes and allow to solidify what does protein supply in complex chemical media? Why is it important to first moisten the applicator swab with sterile broth media? 2. used for most heterotrophic bacteria and fungi How many types of bacteria appear on a successful subculture to a slant? This information is useful in microbial identification, as some species will grow and some will not, depending on the parameters chosen. blood agar, chocolate agar, suppress the growth of unwanted bacteria / allow growth of other bacteria Exactly what is a Mordant in Microbiology? Bacteria were first observed by Anton von Leeuwenhoek in the late 17th century, but didnt become the objects of serious scientific study until the 19th century, when it became apparent that some species caused human diseases. Growth in live animals - e.g., Mycobacterium leprae / armadillos sputum/respiratory samples Subject: Official Notice for all users of Big Bank! The Serratia marcescens cultures were accidently incubated at 37o C instead of 25o C. You The session that this window was launched from, and was using for access to your account, has ended. (b) The force of gravity is the only force acting on the sky diver, so that he is in equilibrium. In laboratory experiments psychologists' concern is not ______________. The question you ask should also be measurable and . Thats the initial step within this subculture process. What is the method most commonly used by microbiologist to obtain pure cultures? Always use aseptic technique to transfer bacteria between growth media. Members of a specific subculture possess: beliefs. describe the difference between the appearance of surface and subsurface colonies in a pour plate; if this is the same species, why do these difference in growth occur, surface colonies often appear large and round and Now I know that HIV can spread _____. o Each tube is poured into a sterile, empty Petri dish, allowed to harden and incubated Why was the spectrophotometer absorbance reset to "zero" without bacteria in the broth? only that specific bacterium to grow. Their small size means you can't go to a zoo or take a safari to study these creatures; you have to raise them yourself. You remove a very small percentage--a subsample--of an existing population of cells and put them in a new medium where they can thrive and procreate. It's never been easier to find and study microbiology flashcards made by students and teachers using Quizlet. Which species is? provide information on the organisms requirement for oxygen. As least 1 ml must be cultured in neonates. o Steam sterilizing (autoclaving); incineration; hazardous waste disposal companies, stock cultures are maintained for routine research, testing, and quality control This question will include one of the key starters: how, what, when, why, where, who or which. 1. Distinction between subculture and culture. Microbiology is the study of organisms too small to see with the naked eye. a clone of bacterial cells growing on a solid culture medium. lysogeny broth: Lysogeny broth (LB) is a nutritionally-rich medium; primarily used for the growth of bacteria. Determine whether each solution is acidic, basic, or neutral. Distinction between subculture and culture. 4. correct o2 level Should auld sessions be forgot, And days of auld lang syne. a nutrient material prepared for the growth of microorganisms, the process of introducing a sample onto a culture medium, the microbes or sample that is placed onto a culture medium to initiate growth, a culture that is made from a sample of a previous culture, a clone of bacterial cells growing on a solid culture medium; theoretically, dilutes bacteria on the surface of an agar plate (Petri dish), o The dilution is made in tubes of melted agar Good for obtaining growth when bacterial numbers are low ; will increase numbers to a detectable level A week later, the following e-mail was delivered to the controller: 3. adjusted pH ( buffers) 5. Please describe in complete sentences and in your own words, the purpose of this experiment. 3. separation tools, a colony arises from a ____ ____ or _____. Wondering what a bacterial colony is? with your instructor regarding how to safely use incinerators or burners to sterilize. What is another word for subculture? 1. nutrients 9. Consider the streak plate subcultures that you simply made. If transport to a microbiology laboratory is not possible the same day, place the blood culture bottle in an incubator at 35-37C with ~5% CO 2 (or in a candle-jar) until transport is possible. - Assortment seeking - Social interaction On the bottom of the plate = Plates are incubated upside down to keep lid condensation from falling onto the agar surface. 2. colonies within agar can't be tested To get isolated colonies from the mixed culture. In this laboratory exercise, Serratia marcescens, a bacteria, will be transferred into a broth culture and Culture. Dont forget to subscribe and hit the bell button so youll always know when our newest new videos are available: https://bit.ly/38X1FPp, A controllable microchemostat can provide an ideal, powerful means to study the growth behavior of microorganisms by improving conventional macroscale chemostat. Make a suspension of cells or spores from the isolate to become stored, preferably utilizing an 18 to round-the-clock old culture grown on solid medium. Where is the label placed on a petri plate? This action is called subculturing or passaging the cells. marcescens, inoculating loop, inoculating needle, incinerators. However, a challenge remains for implementing both continuous growth and active population control of microorganisms at the same time beca . what temperature does agar remain solid at? Liquid growth media are called broths, and gel-like media are called agars. What is a Subculture in Microbiology? Identification and Characterization of Streptococcus pneumoniae. Pick an isolated colony (not mixed with or directly adjacent to another colony) to avoid mixing bacterial types and contaminating the pure culture. or hands by touching either the growth media or the inner surfaces of the culture tube with objects that 8. Apply the concept of aseptic technique and its importance in the field of microbiology. settings. 1. provides isolated colonies on the top of the agar by drag-diluting the colony forming units (CFUs) out across 4 sections of the plate = bacterial culture is only added to the plate one time. This type of subculturing is simply to keep a cell line healthy. Transfer a bacterial culture sample into melted agar tube The outside of the can would be covered in microbes. because different groups of bacteria react differently, 1. blood agar - Masculine traits: aggressiveness, competitiveness and independence. (Section 27.11). Ask a question. Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. o with or without oxygen, grows only a single known species of microorganism, to make a second-level culture from a well-isolated colony, has two or more identified or differentiated species of microorganisms, has had a contaminant or unwanted organism introduced into it, Colony appearance What is subculture? - confident and self-focused. needles. The test also allows differentiating aerobic and obligate anaerobic organisms. In class we did the Quadrant streak (sectioning agar plate into 3 sections). In a hospital setting, contamination of clinical samples may have an 2. deep agar Culture is the collective values, customs, norms, arts, social institutions, and intellectual achievements of a particular society. During the final stages of descent, a sky diver with an open parachute approaches the ground with a constant velocity. 2. incineration method for obtaining pure cultures of bacterial species from mixed microbial populations. 2. macconkey agar e. Metal loops only: Flame the loop to sterilize it. Karen ate at a family barbeque and became ill. She beleives that one of the food items that she ate at the barbeque has ca Treponema pallidum - the syphilis spirochete ; cannot grow on artificial media - media-savvy. Which of the following statements is correct regarding the samples you created? why can't synthetic chemical media be used for fastidious organisms? Subculturing method of fungus. This can be put onto plates or in tubes. In 2013, scientists found that coelacanths are not the closest relative of four-footed amphibians and other animals. When finished, incubate the tubes at approximately 25 o C for 24 to 48 hours. Procedure. The exponential or log phase is a time of . what is a real life example of a mixed culture? Traditionally this has been a matter of culturing isolates on agar slants of suitable media and then subculturing onto fresh slants at regular intervals. In contrast, upright agar tubes are used for stab cultures, which can Outline method 1. 3. 4. subculture. way of air currents. differences occur because of the availability of more nutrients on surface and better dilution of the toxic by-products on the surface than within the medium, Unit 3 Suicide and Nonsuicidal Self-Injury, Unit 5- Child, Older Adult, and Intimate Part, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine, Organizational Behavior: Managing People and Organizations, Jean Phillips, Ricky W. Griffin, Stanley Gully. Failure to confirm your account information will require us to suspend your account until confirmation is made. . S. pneumoniae may occur intracellularly or extracellularly as gram-positive lanceolate diplococci, but can also occur as single cocci or in short chains of cocci. : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), The Methodology of the Social Sciences (Max Weber), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. You sample the broth with a tool, such as a thin metal ring called an inoculation loop. 4. However, some in-person microbiology lab procedures may recommend keeping your Bunsen flame on and working nearby it on the benchtop as you complete this experiment. 7. transport media. most influenced by friends and peers They do not have the identifying characteristics of surface colonies such as edge, surface characteristics, and in some cases, pigment. Whatever the size of the laboratory s stock culture collection, it is important that it is properly maintained. proteins Activity Deliverable Points Adequate blood - broth ratio of 1:10 must be achieved to dilute the effects of inhibitory substances and antimicrobials present in the blood. Subsurface colony = grows inside agar, Scanty growth = low number 6. animal inoculation, test the isolate against known antibodies, cultures and specimens are _______, so proper ____ ____ must be used, 1. steam sterilizing (autoclaving) Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another.For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives.It is, therefore, said to be selective for Gram-positive organisms, and . In most cases, the recommended medium and serum can be purchased from ATCC along with the cell line. 5. The specific formulation of broths and agars can be adjusted to support general microbial growth or to optimize growth of a particular organism. 6. carbohydrate fermentation media a culture that is made from a sample of a previous culture. The Bunsen flame augments our aseptic technique as working near the flame reduces the chances that airborne microorganisms land on the open petri dishes when pour plates are being created. Subculture is used to prolong the life and/or expand the number of cells or microorganisms in the culture. recapping the tubes? 4. The plates should be placed in the incubator upside-down to prevent contamination from condensation, Surface colony = grows on the top of agar Microscopic examination During the steps of the streak plate method, the bacterial culture is, only added directly to the agar plate one time. Salmonella is a member of the family Enterobacteriaceae. a culture that is made from a sample of a previous culture. Subsurface colonies must grow under limited oxygen conditions, which would affect growth and some characteristics. Re-sterilize the loop and then drag the loop through the previously inoculated quadrant to pick up a small fraction of sample to the next quadrant surface. To avoid condensation dripping on the colonies and spreading them everywhere. As the bacteria replicate to higher numbers, the amount of the light passing through the culture will __________ as bacterial cell numbers increase. the tube and obtain a sample by gently touching the surface of the slant where there is A distinct cultural group that exists as an identifiable segment within a larger, more complex society. Microbiology guide to interpreting minimum inhibitory concentration (MIC) This guide provides a detailed explanation of the following concepts which are important in implementing the MIC: The MIC number is the lowest concentration (in g/mL) of an antibiotic that inhibits the growth of a given strain of bacteria. The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population. The light will be __________ by bacterial cells. 3. trypticase soy agar. By simple association, lots of people know that "colony" refers to a group, and "bacteria" refer to a type of microorganism. (a) [H3O+]=1105M;[OH]=1109M\left[\mathrm{H}_3 \mathrm{O}^{+}\right]=1 \times 10^{-5} \mathrm{M} ;\left[\mathrm{OH}^{-}\right]=1 \times 10^{-9} \mathrm{M}[H3O+]=1105M;[OH]=1109M, (b) [H3O+]=1106M\left[\mathrm{H}_3 \mathrm{O}^{+}\right]=1 \times 10^{-6} \mathrm{M}[H3O+]=1106M; [OH]=1108M\left[\mathrm{OH}^{-}\right]=1 \times 10^{-8} \mathrm{M}[OH]=1108M, (c) [H3O+]=1107M\left[\mathrm{H}_3 \mathrm{O}^{+}\right]=1 \times 10^{-7} \mathrm{M}[H3O+]=1107M; [OH]=1107M\left[\mathrm{OH}^{-}\right]=1 \times 10^{-7} \mathrm{M}[OH]=1107M, (d) [H3O+]=1108M\left[\mathrm{H}_3 \mathrm{O}^{+}\right]=1 \times 10^{-8} \mathrm{M}[H3O+]=1108M; [OH]=1106M\left[\mathrm{OH}^{-}\right]=1 \times 10^{-6} \mathrm{M}[OH]=1106M, Several body organs are listed. is the exact chemical composition known in complex chemical media? 3. mannitol salt agar, lactose fermenters and non-lactose fermenters. Microbiology Chapter 3 Flashcards Start studying Microbiology Chapter 3. - Society assigns certain traits/roles to males/ females. - Browsing, Shopping patterns of women who work in paid employment. which classifications of media agars are both selective and differential? Liquid growth media are called broths, and gel-like media are called agars. require high concentrations of co2 for incubation, to separate the bacteria in a sample into individual cells where each cell will then grow into a colony. All Right Reserved. The typical stock culture collection may contain isolates that fall into one or more of the following categories:It is difficult to conceive of a laboratory that does not need stock cultures for at least one of these reasons, even if it is simply a matter of keeping a few reference strains for QC purposes. 1. by using an incompletely sterilized instrument It gets its name from the Danish bacteriologist Hans Christian Gram who first introduced it in 1882, mainly to identify organisms causing pneumonia. o used as a noun - the observable growth; microbes that grow & multiply in a culture medium. what does carbohydrate fermentation media contain? When was the first . They eat, they breathe, they reproduce, they excrete. by leaving the lids off of petri dishes for too long. Why? 8. to maintain and preserve specimens that have to be held for a long period of time before clinical analysis, what animals are living and can have growth in them? c. Remove the loop, flame the mouth of the tube and recap it. As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate. Ideally, you end up spreading the microorganisms so thinly they form tiny, distinct colonies on the agar plate--each sourced from one single microorganism. If you do not wait 10 20 seconds after flame sterilizing the inoculating instruments before - Younger males more positive about their sexuality and inner-selves. 2. from Radboud University NijmegenGraduated 2002Lives in Lausanne, Switzerland2013present, Your email address will not be published. Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology. The Gram staining is one of the most crucial staining techniques in microbiology. the sample is inoculates serially into a series of cooled but still liquid agar tubes so as to dilute the number of cells in each successive tube in the series, 1 out of 4 steps in the pour plate method of isolation technique, 1. the dilution is made in tubes of melted agar, 2 out of 4 steps in the pour plate method of isolation technique, 2. each tube is poured into a sterile, empty petri dish, allowed to harden, and incubated, 3 out of 4 steps in the pour plate method of isolation technique, 3. count colonies (choose plate with 25-250 colonies) - some colonies may develop within the agar, 4 out of 4 steps in the pour plate method of isolation technique, 4. multiply by dilution factor to get the number of cells per ml of sample, advantage to pour plate method of isolation, 3 disadvantages to pour plate method of isolation, 1. dilution errors